High molecular weight (HMW) typing of Clostridium difficile is a subtyping method that enables rapid and low cost surveillance of C. difficile isolates. HMW types are directly correlated to one or more PCR ribotypes.
In summary, HMW typing is a two-step method. First, a HMW profile is determined based on the proteins detected in the 30 to 50 kDa region. For further discrimination within a subset of HMW profiles an optional second step can be performed. Second, based on the presence or absence of certain biomarkers in the 2 to 20 kDa region, the HMW type is determined . For best results it is recommend to analyze the isolates in the second step after performing the standard ethanol/formic acid extraction procedure. The list of PCR ribotypes, with international nomenclature analyzed by HMW typing, can be found under the documentation tab. A full description of the method is published in PLoS ONE1.
This site provides you with an automatic installation package containing all files needed for performing the HMW typing method on your Bruker instrument. Furthermore, it also allows you to generaterun files and upload raw data for automatic analysis of the HMW profiles and HMW types of your isolates.
To start analyzing your samples, download the practical protocol and register for a free 6 month trial period. This website and the HMW type database are curated by the Public Health Agency of Sweden (Folkhälsomyndigheten).